Journal: RSC Advances

Article Title: Tellurium nanoparticles as antimicrobial agents for multi-drug-resistant infections

doi: 10.1039/d5ra02635k

Figure Lengend Snippet: ROS generation assessment using the fluorescent probe H2DCFDA after incubation of (a) bacteria ( S. aureus , E. coli , and P. aeruginosa ) and (b) human keratinocytes and fibroblasts with TeLigNPs. Results are reported as mean values ± SD ( n = 3).

Article Snippet: These nanoparticles exhibited potent antimicrobial activity against E. coli JM109, P. aeruginosa PAO1, and S. aureus ATCC 25923.

Techniques: Incubation, Bacteria

Journal: RSC Advances

Article Title: Tellurium nanoparticles as antimicrobial agents for multi-drug-resistant infections

doi: 10.1039/d5ra02635k

Figure Lengend Snippet: Assessment of E. coli BW25113 membrane integrity following treatment with BioTe or tellurite. (a–c) Representative SEM images of untreated cells (a), BioTe-treated cells (b), and tellurite-treated cells (c). Red arrows indicate membrane perforations in treated samples. Scale bars: 1 μm. (d–f) PI staining highlights membrane-compromised cells in the untreated control (d), BioTe-treated (e), and tellurite-treated (f) groups. (g–i) SYTO 9 staining shows all cells in untreated (g), BioTe-treated (h), and tellurite-treated (i) samples. (j–l) Bright-field images of the corresponding samples. Scale bars for fluorescence and bright-field images: 10 μm. (m) β-Galactosidase activity in culture supernatants of E. coli BL21(DE3) overexpressing lacZ , treated with BioTe (3× MIC), tellurite (3× MIC), or left untreated for 1 hour. Samples were analyzed via SEM, fluorescence staining, and β-galactosidase activity measurement. Data represent mean ± SD ( n = 3). Asterisks indicate statistically significant differences ( p < 0.05).

Article Snippet: These nanoparticles exhibited potent antimicrobial activity against E. coli JM109, P. aeruginosa PAO1, and S. aureus ATCC 25923.

Techniques: Membrane, Staining, Control, Fluorescence, Activity Assay

Journal: RSC Advances

Article Title: Tellurium nanoparticles as antimicrobial agents for multi-drug-resistant infections

doi: 10.1039/d5ra02635k

Figure Lengend Snippet: Antibacterial and antibiofilm activity of Te and Te–SO NNs against E. coli . (a) Colony count variation and (b) antibacterial efficacy at different concentrations of Te and Te–SO NNs. (c) Fluorescence microscopy images of E. coli stained with SYTO 9 (live cells, green) and PI (membrane-compromised cells, red) following treatment with Te and Te–SO NNs (100 μg mL −1 ). SEM images depicting morphological changes in (d) planktonic E. coli cells and (e) E. coli biofilms after exposure to Te or Te–SO NNs (100 μg mL −1 ).

Article Snippet: These nanoparticles exhibited potent antimicrobial activity against E. coli JM109, P. aeruginosa PAO1, and S. aureus ATCC 25923.

Techniques: Activity Assay, Fluorescence, Microscopy, Staining, Membrane

Journal: RSC Advances

Article Title: Tellurium nanoparticles as antimicrobial agents for multi-drug-resistant infections

doi: 10.1039/d5ra02635k

Figure Lengend Snippet: In vivo therapeutic efficacy of Te NNs/PVA hydrogel on wounds co-infected with S. aureus and E. coli . (a) Representative images of wound healing progression in rats treated with PBS, PVA hydrogel, and Te NNs/PVA hydrogel at days 0, 4, 7, 10, and 14 (scale bar: 1 cm). (b) Bacterial culture images obtained from skin tissue samples across different treatment groups over the same period. (c) Quantitative analysis of wound healing rates from day 4 to day 14. (d) Residual bacterial counts at wound sites for each treatment group. Data are presented as mean ± standard deviation ( n = 5 per group); * p < 0.05, ** p < 0.01, compared to the Te NNs/PVA group.

Article Snippet: These nanoparticles exhibited potent antimicrobial activity against E. coli JM109, P. aeruginosa PAO1, and S. aureus ATCC 25923.

Techniques: In Vivo, Drug discovery, Infection, Standard Deviation

Journal: RSC Advances

Article Title: Tellurium nanoparticles as antimicrobial agents for multi-drug-resistant infections

doi: 10.1039/d5ra02635k

Figure Lengend Snippet: Histological analysis of skin tissue from S. aureus and E. coli co-infected wounds following treatment with different dressings. (a) Hematoxylin and eosin (H&E) stained images and (b) Masson's trichrome stained images, illustrating the extent of bacterial infection and tissue regeneration after 7 and 14 days of treatment. Scale bar: 100 μm. Yellow arrows indicate neutrophils, while blue arrows highlight fibroblasts.

Article Snippet: These nanoparticles exhibited potent antimicrobial activity against E. coli JM109, P. aeruginosa PAO1, and S. aureus ATCC 25923.

Techniques: Infection, Staining